Ribosomal protein S8



Ribosomal protein S8 (RPS8) is one of the 22 proteins which belong to the small subunit of the bacterial ribosome. It binds to 16S RRNA and interacts with spc operon mRNA. In archaea the protein is named S8E (RPS8E). The images at the left and at the right correspond to one representative RPS8, i.e. the crystal structure of Ribosomal protein S8 from Thermus thermophilus (2zm6, chain H).

3D Structures of Ribosomal protein S8
Update June 2011

RPS8
1an7 – TtRPS8 - Thermus thermophilus

1sei – RPS8 – Geobacillus stearothermophilus

2kcp, 2kcy – RPS8E – Methanothermobacter thermaututrophicus – NMR

2kco – RPS8E – Sulfolobus solfataricus - NMR

S8 in small ribosomal subunit
2zm6, 1n36, 1j5e, 3oge, 3ogy, 3ohc, 3ohd, 3ohy, 3oi0, 3oi2, 3oi4, 3oto - TtRPS8 in 30S ribosomal subunit+THX+16S RRNA – Thermus thermophilus

2vqe, 2vqf, 2uuc, 2e5l, 2uu9, 2uua, 2uub, 3pyn, 3pyq, 3pys, 3pyu - TtRPS5 in 30S ribosomal subunit+16S RRNA+RNA fragment

3kc4 – EcRPS8 in 30S ribosomal subunit - Escherichia coli

3i1m, 3i1o, 3df1, 3df3, 2vho, 2vhp, 2avy, 2aw7, 1pnx, 3oaq, 3oar, 3ofa, 3ofb, 3ofo, 3ofp, 3ofx, 3ofy, 3or9, 3ora - EcRPS8 in 30S ribosomal subunit+16S RRNA

1i94, 1fka - TtRPS8 in 30S ribosomal subunit+16S RRNA

1p87, 1p6g - EcRPS8 in 30S ribosomal subunit+16S RRNA – Cryo-EM

3bbn - RPS8 in 30S ribosomal subunit+16S RRNA – Cryo-EM – spinach

S8 in small + large ribosomal subunit
1ml5 - EcRPS8 in 30S ribosomal subunit+50S ribosomal subunit+THX+chain release factor 2+P-site tRNA+tRNA Phe+mRNA fragment+23S RRNA+5S RRNA+16S RRNA - EM

S8 in small ribosomal subunit+ chain release factor
3mr8, 3ms0 – TtRPS8 in 30S ribosomal subunit+THX+chain release factor 1+P-site tRNA+mRNA+16S RRNA

3d5a, 3d5c - TtRPS8 in 30S ribosomal subunit+THX+chain release factor 1+P-site tRNA+E-site tRNA+mRNA +16S RRNA

2b64 - TtRPS8 in 30S ribosomal subunit+THX+P-site tRNA Phe+E-site tRNA Phe+mRNA +16S RRNA+chain release factor 1

2b9m - TtRPS8 in 30S ribosomal subunit+THX+P-site tRNA Phe+E-site tRNA Phe+mRNA +16S RRNA+chain release factor 2

2x9r, 2x9t, 2wh1, 2wh3 - TtRPS8 in 30S ribosomal subunit+THX+chain release factor 2+P-site tRNA+mRNA +16S RRNA

3f1e, 3f1g - TtRPS8 in 30S ribosomal subunit+THX+chain release factor 2+P-site tRNA+E-site tRNA +16S RRNA

S8 in small ribosomal subunit+tRNA+mRNA
2hgi - TtRPS8 in 30S ribosomal subunit+16S RRNA+tRNA Phe+tRNA fMet

2hgp - TtRPS8 in 30S ribosomal subunit+16S RRNA+tRNA Phe+mRNA

2i2p, 2i2u - EcRPS8 in 30S ribosomal subunit+16S RRNA+tRNA Phe+mRNA

2hgr - TtRPS8 in 30S ribosomal subunit+16S RRNA+tRNA Phe+tRNA fMet+mRNA

1yl4 – TtRPS8 in 30S ribosomal subunit+ tRNA Phe+16S RRNA+THRS mRNA operator

1pns - EcRPS8 in 30S ribosomal subunit+ tRNA Phe+16S RRNA+mRNA

1xnq, 1xnr - TtRPS8 in 30S ribosomal subunit+THX+mRNA+tRNA anticodon+16S RRNA

S8 in small ribosomal subunit+A-site tRNA
2uxd, 2uxc - TtRPS8 in 30S ribosomal subunit+THX+A-site mRNA CGGG+tRNA anticodon+16S RRNA

1n32, 1n33 - TtRPS8 in 30S ribosomal subunit+THX+A-site mRNA fragment+tRNA anticodon+16S RRNA

1n34 - TtRPS8 in 30S ribosomal subunit+THX+A-site mRNA fragment +16S RRNA

1xmo, 1xmq - TtRPS8 in 30S ribosomal subunit+THX+A-site mRNA+tRNA anticodon+16S RRNA

2uxb - TtRPS8 in 30S ribosomal subunit+THX+A-site mRNA GGGU+tRNA anticodon+16S RRNA

S8 in small ribosomal subunit+P-site tRNA
3i8g - TtRPS8 in 30S ribosomal subunit+P-site tRNA+mRNA +16S RRNA

3i8h, 3i9b, 3i9d - TtRPS8 in 30S ribosomal subunit+THX+P-site tRNA+mRNA +16S RRNA

1ibk - TtRPS8 in 30S ribosomal subunit+THX+P-site mRNA fragment +16S RRNA

2wwl - EcRPS8 in 30S ribosomal subunit+Poly-Ala chain+P-site tRNA+mRNA +16S RRNA - Escherichia coli

3i1q - EcRPS8 in 30S ribosomal subunit+P-site tRNA hairpin+mRNA

3i1s, 3i1z, 3i21 - EcRPS8 in 30S ribosomal subunit+P-site tRNA hairpin+mRNA +16S RNA

S8 in small ribosomal subunit+A-site tRNA+P-site tRNA
1ibl, 1ibm - TtRPS8 in 30S ribosomal subunit+THX+A-site mRNA fragment+P-site mRNA fragment+tRNA anticodon+16S RRNA

S8 in small ribosomal subunit+P-site tRNA+E-site tRNA
2ow8 - TtRPS8 in 30S ribosomal subunit+THX+P-site tRNA Phe+E-site tRNA+mRNA +16S RRNA

2b9o - TtRPS8 in 30S ribosomal subunit+THX+P-site tRNA Phe+E-site tRNA Phe+mRNA +16S RRNA

2qnh - TtRPS8 in 30S ribosomal subunit+THX+P-site tRNA+E-site tRNA+mRNA +16S RRNA

2wdg, 2wdh, 2wdk, 2wdm - TtRPS8 in 30S ribosomal subunit+THX+P-site tRNA+E-site tRNA+mRNA +16S RRNA+tRNA Phe

S8 in small ribosomal subunit+A-site tRNA+P-site tRNA+E-site tRNA
2gy9, 2gyb - EcRPS8 in 30S ribosomal subunit+P-site tRNA+A-site tRNA+E-site tRNA+mRNA +16S RRNA – Cryo-EM

1jgo, 1jgp, 1jgq, 1gix - TtRPS8 in 30S ribosomal subunit+P-site tRNA+A-site tRNA+E-site tRNA+tRNA Phe+mRNA +16S RRNA

3e1a, 3e1c - EcRPS8 in 30S ribosomal subunit+P-site tRNA+A-site tRNA+E-site tRNA+mRNA +16S RRNA

S8 in small ribosomal subunit + antibiotics
1i95, 1i97, 2hhh, 2f4v - TtRPS8 in 30S ribosomal subunit+16S RRNA+antibiotic

1hnw, 1hnx, 1hnz,  1fjg - TtRPS8 in 30S ribosomal subunit+16S RRNA+mRNA fragment+antibiotic

2qbb, 2qb9, 1vs5, 1vs7, 2qan, 2qal,  2qou,  2qow,  2qoy,  2qp0,  2z4k,  2z4m - EcRPS8 in 30S ribosomal subunit+16S RRNA+antibiotic

2j00, 2j02 - TtRPS8 in 30S ribosomal subunit+16S RRNA+paromomycin+P-site tRNA fMet+E-site tRNA Phe+mRNA

3knj, 3knh - TtRPS8 in 30S ribosomal subunit+P-site tRNA+A-site tRNA+E-site tRNA+mRNA +16S RRNA+viomycin

3knl, 3knn - TtRPS8 in 30S ribosomal subunit+P-site tRNA+A-site tRNA+E-site tRNA+mRNA +16S RRNA+capreomycin

S8 in small ribosomal subunit + ribosome recycling factor
2qbd, 2qbf - EcRPS8 in 30S ribosomal subunit+16S RRNA+ribosome recycling factor

3r8n, 3r8o – EcRPS7 in 30S ribosomal subunit+16S RRNA+ribosome recycling factor + mRNA + tRNA

2qbh, 2qbj - EcRPS8 in 30S ribosomal subunit+16S RRNA+ribosome recycling factor+gentamicin

2v46, 2v48 - TtRPS8 in 30S ribosomal subunit+16S RRNA+ribosome recycling factor+P-site tRNA Phe+mRNA+tRNA fMet

S8 in small ribosomal subunit + elongation factor
2wri, 2wrk, 2xsy, 2xuy - TtRPS8 in 30S ribosomal subunit+THX+P-site tRNA+mRNA +16S RRNA+elongation factor G

2wrn, 2wrq - TtRPS8 in 30S ribosomal subunit+THX+A-site tRNA+mRNA +16S RRNA+elongation factor TU-4

3fic - TtRPS8 in 30S ribosomal subunit+THX+A-site tRNA+P-site tRNA+E-site tRNA+mRNA codon+16S RRNA+elongation factor TU-A

3izv – EcRPS8 in 30S ribosomal subunit+A/T-site tRNA Phe +16S RRNA+elongation factor TU

3izw – EcRPS8 in 30S ribosomal subunit+A-site tRNA Phe +mRNA +16S RRNA+elongation factor TU

2y0u, 2y0w, 2y0y,  2y10,  2y12,  2y14,  2y16,  2y18 - TtRPS8 in 30S subunit+THX+A-site tRNA+E-site tRNA+mRNA codon+16S RRNA + elongation factor TU

3fih - EcRPS8 in 30S ribosomal subunit+A-site tRNA Phe+P-site tRNA+mRNA +16S RRNA+elongation factor TU

3huw, 3huy - TtRPS8 in 30S ribosomal subunit+THX+tRNA-Met+mRNA +16S RRNA+elongation factor P

2xqd - TtRPS8 in 30S ribosomal subunit+THX+E-site tRNA+mRNA +16S RRNA+elongation factor TU-B

S8 in small ribosomal subunit + translation initiation factor
1i96 - TtRPS8 in 30S ribosomal subunit+THX+IF3+16S RRNA

1hr0 - TtRPS8 in 30S ribosomal subunit+THX+IF+16S RRNA

S8 in small ribosomal subunit + various proteins
3kiq, 3kis, 3kiu, 3kix - TtRPS8 in 30S ribosomal subunit+toxin RelE+16S RRNA

1voq, 1vos, 1vov, 1vox, 1voz - EcRPS8 in 30S ribosomal subunit+protein Y+16S RRNA

2xzm, 2xzn - RPS8 in 40S subunit+18S RRNA+RACK1 - Tetrahymena thermophila

2xfz, 2xg1 - TtRPS8 in 30S ribosomal subunit+colicin E3+E-site tRNA+mRNA+16S RRNA

3j00 – EcRPS8 + 16S RRNA + FTSQ + apolipoprotein A-I

S8 in part of small ribosomal subunit
1qd7 – TtRPS8+TtRPS4+TtRPS6+TtRPS7+TtRPS5+TtRPS15+TtRPS17+TtRPS20+16S RRNA fragment

3iy8 - EcRPS8+EcRPS5+EcRPS6+EcRPS9+EcRPS9 EcRPS11+EcRPS12+EcRPS15+EcRPS16+EcRPS17+EcRPS18+Leishmania taretolae mRNA

1s03 - EcRPS8+spc operon mRNA

1i6u - RPS8+16S RRNA fragment – Methanococcus jannaschii

1emi - TtRPS8+16S RRNA